Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler.
نویسندگان
چکیده
We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.
منابع مشابه
Rapid characterization and engineering of natural product biosynthetic pathways via DNA assemblerw
We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unpre...
متن کاملManipulating natural product biosynthetic pathways via DNA assembler.
DNA assembler is an efficient synthetic biology method for constructing and manipulating biochemical pathways. The rapidly increasing number of sequenced genomes provides a rich source for discovery of gene clusters involved in synthesizing new natural products. However, both discovery and economical production are hampered by our limited knowledge in manipulating most organisms and the corresp...
متن کاملDNA assembler, an in vivo genetic method for rapid construction of biochemical pathways
The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae. We show that DNA assembler can rapidly assemble a functional D-xylose utilization pathway...
متن کاملDetermination of Material Flows in a Multi-echelon Assembly Supply Chain
This study aims to minimize the total cost of a four-echelon supply chain including suppliers, an assembler, distributers, and retailers. The total cost consists of purchasing raw materials from the suppliers by the assembler, assembling the final product, materials transportation from the suppliers to the assembler, product transportation from the assembler to the distributors, product transpo...
متن کاملIn silico identification of miRNAs and their target genes and analysis of gene co-expression network in saffron (Crocus sativus L.) stigma
As an aromatic and colorful plant of substantive taste, saffron (Crocus sativus L.) owes such properties of matter to growing class of the secondary metabolites derived from the carotenoids, apocarotenoids. Regarding the critical role of microRNAs in secondary metabolic synthesis and the limited number of identified miRNAs in C. sativus, on the other hand, one may see the point how the characte...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Molecular bioSystems
دوره 7 4 شماره
صفحات -
تاریخ انتشار 2011